In Vitro Polarization of Murine Macrophage
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Macrophages are tissue-resident professional phagocytes, which play an important role in immune-regulation. Activated macrophages are routinely classified into two different types: M1-macrophages (classic activation) and M2- macrophages (alternative activation), which perform pro- and anti-inflammatory active respectively.
In vitro polarization of macrophages is achieved by stimulation in the presence of appropriate activation factors. Here we describe a comprehensive protocol to obtain specific macrophage subtypes through in vitro polarization from murine bone marrow derived and peritoneal macrophages.