maleimide conjugation

The carbohydrate moiety on an antibody is located at the hinge region of the Fc domain, with a sufficient distant from the variable region that regulates antigen interaction. Therefore, conjugation involving carbohydrate moiety is unlikely to impair the antigen binding affinity and could potentially serves as an excellent alternative for site-specific antibody drug conjugate (ADC) generation. https://www.creative-biolabs.com/adc/carbohydrate-based-conjugation.htm