Total Protein Extraction by TRIzol

Extraction of DNA, RNA and proteins is a common procedure involving in molecular approaches for the studies of genome, transcriptome and proteome of biological samples. Although designed for the extraction of DNA, RNA and proteins, TRIzol is utilized most frequently for the extraction of nucleic acids, while RIPA is the most widely used agent for protein extraction. Simultaneous extraction of DNA, RNA, and proteins is sample-saving, and coordinated analysis of an individual sample is crucial for accurate correlations between genomic aberrations and their consequences on the transcriptome and proteome. Thus, we provide a detailed protocol describing extraction of total protein following TRIzol isolation of nucleic acids.